mobility shift assay การใช้

• An electrophoretic mobility shift assay can assess whether a structure incorporates all desired strands.
• By using electrophoretic mobility shift assay ( EMSA ), the activation profile of transcription factors can be detected.
• Historically, the experimental techniques of choice to discover and analyze DNA binding sites have been the DNAse footprinting assay and the Electrophoretic Mobility Shift Assay ( EMSA ).
• It has been used in the electrophoretic mobility shift assay and p50 and title comp preincubated in assay buffer, pH 7.5 ( room temp ., 5 min ).
• Alternatively, K d can be calculated from a gel mobility shift assay in which the same purified protein is incubated with serial dilutions of gel-purified, 32P-end-labelled target oligonucleotide.
• Assays are created that combine reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluorescence microscopy to silence the T6SS gene cluster by the histone-like nucleoid structuring H-NS protein.
• Proteins which associate with the promoter can be identified using an electrophoretic mobility shift assay ( EMSA ), and the effects of inclusion or exclusion of the proteins with the mutagenized promoters can be assessed in the assay.
• Capsulin was translated in a rabbit reticulocyte lysate in the presence and absence of the widely expressed bHLH protein E12 and performed gel mobility shift assays with several E-box sequences as probes to test the protein's DNA binding activity.
• FANCA binds to both single-stranded ( ssDNA ) and double-stranded ( dsDNA ) DNAs; however, when tested in an electrophoretic mobility shift assay, its affinity for ssDNA is significantly higher than for dsDNA . FANCA also binds to RNA with a higher affinity than its DNA counterpart.